Thursday, May 28th, 2020


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miR-138-5p Promotes Proliferation of Human Melanoma Cells by Inhibiting hTERT Expression
Authors:  Tingting Ye, M.S., Yexuzi Li, B.S., Junying Ye, B.S., and Chengzhong Zhang, B.S.
  Objective: Melanoma is one of the most aggressive and fatal forms of skin cancer. MicroRNAs (miRNAs), a series of small noncoding RNAs, function through translation repression and/or mRNA degradation. In the present study we aimed to explore the potential role and underlying mechanism of miR-138 in the regulation of melanoma development.
Study Design:
We suggested that miR-138 expression was strongly upregulated in melanoma tissues and human melanoma cells by comparison with their corresponding controls.
Specific overexpression of miR-138-5p promoted Me45 melanoma cell proliferation. By contrast, specific knockdown of miR-138 prevented its proliferation. Bioinformatics analysis indicated that miR-138-5p potentially targets the 3’-untranslated region (3’-UTR) of human telomerase reverse transcriptase (hTERT), which was confirmed by luciferase activity assay. Further, this study revealed that miR-138-5p regulated the proliferation of human melanoma cells by directly regulating hTERT expression as shown by the result that knockdown of hTERT reversed the promotive effects of miR-138-5p on proliferation of human melanoma cells.
Taking all these results together, this study demonstrated that miR-138-5p acts as a carcinogenesis factor by targeting hTERT during human melanoma development.
Keywords:  hTERT, malignant melanoma, melanoma, microRNA, miRNA, miR-138-5p, proliferation, skin cancer, skin neoplasms
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